Whole Exome Sequencing (WES) enables researchers to focus on the genes most likely to affect disorder or phenotype by selectively sequencing the coding regions of a genome. Mice, Mus musculus, are important model organisms for human disease research and drug development. Novogene’s mouse whole exome sequencing (mWES) empowers you to identify causative mutations in mice with phenotypes, which may directly lead to the discovery of human disease genes.Service Specifications Demo Reports
- Drug development
- Model research on human health and disease
- Unsurpassed data quality: We guarantee a Q30 score ≥80%, exceeding Illumina’s official guarantee of ≥75%. See our data example.
- Accurate variant calling with longer read length up to 150 bp.
- Extraordinary informatics expertise: Novogene uses its cutting-edge bioinformatics pipeline and internationally recognized best-in-class software to provide customers with publication-ready data.
|Sample Type||Amount (Qubit®)||Purity|
|Genomic DNA||≥400 ng||OD260/280=1.8-2.0;|
|Genomic DNA from FFPE *||≥0.8 μg|
Sequencing Parameters and Analysis Contents
|Platform||Illumina Novaseq 6000|
|Read length||Paired-end 150 bp|
|Recommended sequencing depth||effective sequencing depth above 50× (6G)|
|Standard analysis||Data Quality Control|
|Alignment with reference genome, statistics of sequencing depth and coverage|
|SNP and InDel calling, annotation and statistics|
|Somatic SNP/InDel/CNV calling, annotation and statistics (paired tumor samples)|
Sample Quality Control
Library Quality Control
Data Qualtiy Control
Exome Capture & Library Preparation
Sequencing error rate distribution
Note: The x-axis represents position in reads, and the y-axis represents the average error rate of bases of all reads at a position.
GC content distribution
Note: The x-axis is position in reads, and the y-axis is percentage of each type of bases (A, T, G, C); different bases are distinguishable by different colors.
Sequencing depth & coverage distribution
Note: Average sequencing depth (bar plot) and coverage (dot-line plot) in each chromosome. The x-axis represents chromosome; the left y-axis is the average depth; the right y-axis is the coverage (proportion of covered bases).
Heatmap of significantly mutated genes